DEVELOPMENT OF A SIZE-BASED SORTING AND BIOMARKER-SPECIFIC MICROFLUIDIC SYSTEM FOR THE ANALYSIS OF EXTRACELLULAR VESICLES

CEA-LETI / DTBS & SYMMES

Extracellular Vesicles (EVs) are nanoscale lipid particles secreted by cells and actively participating to intercellular communication. They have received increasing attention over the past decade as non-invasive biomarkers easily available from liquid biopsy, and they are considered for diagnostic applications. Isolation of such biomarkers however remains challenging mainly due to EVs heterogeneity, especially in size, and biofluids complexity. New methods are required to perform simple, efficient and reproducible EVs isolation. In this PhD thesis, a coupled approach is proposed, based on an aptamer-supported capture of EVs, combined with a Deterministic Lateral Displacement (DLD) size sorting system, in order to demonstrate EVs fractionation according to their protein expression.

PhD student funded by ARCANE : Marie GAILLARD

Isolation of extracellular vesicles subpopulations by combination of capture by aptamer-coated bead microcarriers with microfluidic size-sorting, M. Gaillard, F. Boizot, C. Raillon, V. Agache, A. Thuaire and Y. Roupioz, MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 2021, 683-684

Integration of DLD modules on a microfluidic platform for the fractionation of viral particles, N. Sarrut-Rio, M. Gaillard, F. Boizot, P. Laurent, M. Cubizolles and A.Thuaire, Proceedings of µ-TAS conference, 2021, 1191-1192

Deterministic lateral displacement for the sorting of extracellular vesicles from complex biological samples, M. Gaillard, N. Sarrut-Rio, L. Virot, F. Boizot, N. Verplanck, C. Raillon, V. Agache, Y. Roupioz and A. Thuaire, MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 2020,1075 - 1076

Biosensing extracellular vesicles: contribution of biomolecules in affinity-based methods for detection and isolation, M. Gaillard, A. Thuaire, G. Nonglaton, V. Agache, Y. Roupioz, and C. Raillon, Analyst, 2020, 145, 1997-2013