Solid-state NMR enables the investigation of biological systems within their native context, e.g. lipid membranes, viral capsid assemblies, and cells. However, such investigations often suffer from low sensitivity due to low concentrations. Using dynamic nuclear polarization (DNP), the experimental time can be reduced by orders of magnitude, broadening the application scope. In this review, we outline strategies and present an overview of investigations where DNP has led to unique insights.
Biomolecular and Biological Applications of Solid-state NMR with Dynamic Nuclear Polarization Enhancement, W. Y. Chow, G. De Paëpe, S. Hediger, Chem. Rev. 2022, 122, 9795-9847. DOI: 10.1021/acs.chemrev.1c01043.